Abstract—In the work a planar topology for nanoelectronic transducer based on the enzyme glucose oxidase is developed. An experimental study of the possibility of forming nanogaps in planar nanostructures by electromigration is carried out. A technique of implementation of planar nanostructures for nanoelectronic biosensor based on the methods of electron-beam lithography is developed. A technological map of manufacturing of nanogaps between metal electrodes by electromigration of metal atoms on the basis of a computer-controlled process of breaking metal nanowires is developed and realized. The method of the chemical modification of silicon dioxide surface by epoxysilane was developed and the immobilization of the glucose oxidase enzyme on the surface of planar nanostructure for the nanoelectronic transducer through the linker molecules was realized. The registration procedure of the biochemical signals was developed. A change in the functional state of the immobilized ferment of the glucose oxidase enzyme with the presence (oxidation) of glucose in the test solution was demonstrated.
Index Terms—Biosenor, nanotransistor, nanostructures, enzymatic electrochemical sensors, enzymatic activity, environmental monitoring.
Kolesov V. V. and Krupenin S. V. are with Institute of Radioengineering & Electronics, Russian Academy of Sciences, Moscow, Russia (e-mail: kvv@cplire.ru; krupenin@cplire.ru).
Soldatov E. S. is with Faculty of Physics, Moscow State University, Moscow, Russia (e-mail: esold@phys.msu.ru)
Reshetilov A. N. is with Institute of Biochemistry and Physiology of microorganisms, Russian Academy of Sciences, Moscow, Russia (e-mail: anatol@ibpm.pushchino.ru)
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Cite:Kolesov V. V., Krupenin S. V., Soldatov E. S., and Reshetilov A. N., "Biosensor on the Basis of Planar Nanostructure with Built-In Enzymatic Molecular Complexes," International Journal of Materials, Mechanics and Manufacturing vol. 1, no. 2, pp. 117-120, 2013.
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